OSD Pilot 2012 - Crete

Monitoring Station: M3A

Report: OSD – HCMR contribution

HCMR Sampling organizers:

Dr. Paraskevi N. Polymenakou (microbiology)

Dr. Giorgos Kotoulas (genetics)

Dr. C. Fragoulis (zooplankton, organizer of the sampling at monitoring station M3A)

Vessel: R/VPhilia

People on board:

A. Krystallas, technician

C. Christakis, MSc student

Date of sampling: 20 June 2012

Time of sampling: 12:00 – 13:00 local time

Sampling depths:

2 m, 10 m, 20 m, 50 m, 75 m, 100 m, 120 m.

CTD was deployed on site before sampling to record the vertical profiles of temp, salinity, oxygen, conductivity, chl.

A total of 7 depths were sampled for nutrients, chlorophyll and phytoplankton measurements.

The following five sampling depths were chosen for DNA analysis:

The surface at 2 m to use it as control sample since it is characterized by extremely low concentrations of nutrients and the depths of 50 m, 75 m, 100 m and 120 m where a deep chlorophyll maximum is observed this period (usually this period DCM is observed at a depth of 100 m).

800 ml – 1000 ml of water was filtered through 0.22 um, 47 mm, polycarbonate filters (OSMONICS Inc.), and a total of four replicate samples for each sampling depth. In total we have collected 20 samples (5 sampling depths x 4 replicates). (Unfortunately Millipore Sherivex filters did not arrive on time in order to use them for this sampling.)

On Wednesday 13 of June 2012 (1 week before the OSD-June) a more detailed sampling of the water column was performed. Samples for flow cytometry, chlorophylla, POC, phyto- and zooplankton, and chlorophyll size fraction were collected. In addition sediment samples from the same station using a multi-corer system (enables the collection of samples with undisturbed surface) were also collected.