Monitoring Station: M3A
Report: OSD – HCMR contribution
HCMR Sampling organizers:
Dr. Paraskevi N. Polymenakou (microbiology)
Dr. Giorgos Kotoulas (genetics)
Dr. C. Fragoulis (zooplankton, organizer of the sampling at monitoring station M3A)
People on board:
A. Krystallas, technician
C. Christakis, MSc student
Date of sampling:
20 June 2012
Time of sampling: local time
2 m, 10 m, 20 m, 50 m, 75 m, 100 m, 120 m.
CTD was deployed on site before sampling to record the vertical profiles of temp, salinity, oxygen, conductivity, chl.
A total of 7 depths were sampled for nutrients, chlorophyll and phytoplankton measurements.
The following five sampling depths were chosen for
The surface at 2 m to use it as control sample since it is characterized by extremely low concentrations of nutrients and the depths of 50 m, 75 m, 100 m and 120 m where a deep chlorophyll maximum is observed this period (usually this period DCM is observed at a depth of 100 m).
800 ml – 1000 ml of water was filtered through 0.22 um, 47 mm, polycarbonate filters (OSMONICS Inc.), and a total of four replicate samples for each sampling depth. In total we have collected 20 samples (5 sampling depths x 4 replicates). (Unfortunately Millipore Sherivex filters did not arrive on time in order to use them for this sampling.)